小雪的13又嫩又紧又多水,湿润手指揉捏调教H女友,国产欧美日韩a片免费软件,国产精品亚洲一区二区三区在线

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>人腫瘤細(xì)胞、癌細(xì)胞>CRL-2549Panc 03.27細(xì)胞, 人胰腺癌細(xì)胞

Panc 03.27細(xì)胞, 人胰腺癌細(xì)胞

簡(jiǎn)要描述:Panc 03.27細(xì)胞, 人胰腺癌細(xì)胞
原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件!

  • 產(chǎn)品型號(hào):CRL-2549
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2025-04-28
  • 訪  問  量:1436

產(chǎn)品分類

Product Category

詳細(xì)介紹

Panc 03.27細(xì)胞, 人胰腺癌細(xì)胞

ATCC® Number:CRL-2549™    Price:$417.00
Designations:Panc 03.27Depositors:EM JaffeeBiosafety Level:1Shipped:frozenMedium & Serum:See PropagationGrowth Properties:adherentOrganism:Homo sapiens (human)Morphology:epithelial Source:Organ: pancreas Disease: adenocarcinomaCellular Products:cytokeratins 7 and 18Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.Panc 03.27細(xì)胞, 人胰腺癌細(xì)胞Tumorigenic:YesOncogene:K-ras + (wild-type)Antigen Expression:MHC class I +; MHC class II - [50655] Blood type A; Rh+DNA Profile (STR):Amelogenin: X CSF1PO: 10,11 D13S317: 11,12 D16S539: 12 D5S818: 12,13 D7S820: 8,13 THO1: 6 TPOX: 8,9 vWA: 16,17Age:65 yearsGender:femaleEthnicity:WhiteComments:Panc 03.27 is a pancreatic adenocarcinoma epithelial cell line derived in 1995 from a primary tumor removed from the head-of-the-pancreas of a female with pancreatic adenocarcinoma.The cells have a reported plating efficiency of 80%.Propagation:ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 10 Units/ml human insulin, 85%; fetal bovine serum, 15%Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°CSubculturing:Protocol:                               Remove and discard culture medium.Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.Add appropriate aliquots of the cell suspension to new culture vessels.Incubate cultures at 37C.Subc*tion Ratio: A subc*tion ratio of 1:3 to 1:4 is recommended Medium Renewal: Every 2 to 3 daysPreservation:Freeze medium: culture medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phaseDoubling Time:37.5 hrsRelated Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001recommended serum:ATCC 30-20200.25% (w/v) Trypsin - 0.53 mM EDTA in Hank' BSS (w/o Ca++, Mg++):ATCC 30-2101Cell culture tested DMSO:ATCC 4-XReferences:50655: Jaffee EM, et al. Development and characterization of a cytokine-secreting pancreatic adenocarcinoma vaccine from primary tumors for use in clinical trials. Cancer J. Sci. Am. 4: 194-203, 1998. PubMed: 9612602

















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
人妻好久没做被粗大迎合| 免费观看片的APP下载| 精品人妻无码专区在中文字幕| 销魂美女图库| 性少妇tubevⅰdeos高清 | 天堂资源中文最新版在线一区 | 国模冰莲极品自慰人体| 绝伦の上司に一晚人妻| 18禁美女挤奶视频免费观看| JIZZ中国老师高潮喷水| 抖音无限次短视频老司机| 日韩av无码免费播放| 五夫一妻的幸福生活| H高潮娇喘抽搐A片国产麻豆| 公交车上内裤滑进去了会怎么样| 小秘书夹得真紧好爽h调视频| 小悔的性荡生活| 亚洲和欧洲一码二码区别在| 少妇的肉体AA片免费| 国产亚洲成av人片在线观黄桃| 亚洲大码熟女在线观看| 激情国产一区二区三区四区小说| 日本不卡一区二区三区| 太大太长太粗太久太硬了| 国产精品久久久久精品艾秋 | 四虎成人精品无码永久在线| 亚洲欧美日韩一区在线观看| 乌克兰极品少妇XXXX做受| 岳故意装睡让我进去| 批日出水免费观看40分钟| ASS白嫩白嫩的PIC| 色蜜桃久久夜色精品国产| 灭蟑螂土办法| 大香煮伊区一二三四区2021| 美女100%裸体无内衣内裤| 黑人40厘米全部进去a片| 久久www香蕉免费人成| 国产同事露脸对白在线视频| 顶级少妇倣爱A片XXX| 日本japanese丰满少妇| 人妻精品久久无码区洗澡|